Age-Defiance Activating Serum

The N1O1 dual-chamber serum keeps the two formulas separate until use. Mix them in your palm, then apply to the face and neck.

1 fl oz / 30 mL · dual-chamber bottle

Choose your bottles

1 bottle · $119.98 today

$75

Free shipping over $75 in the continental US.

Meet the people behind your routine

  • Developed by Dr. Nathan Bryan
  • Dual-chamber serum
  • Mix at application

Two chambers. One fresh application.

The N1O1 dual-chamber serum keeps the two formulas separate until use. Mix them in your palm, then apply to the face and neck.

Mix at application

The two-chamber design keeps the formulas separate until you dispense them.

A focused step

The original product page highlights vitamin C, vitamin E and sodium nitrite.

Layer your routine

Allow 1–2 minutes for absorption before applying other products.

Nitric Oxide Activating Age-Defiance Serum

Dispense. Mix. Apply.

Build your routine, step by step. Follow the directions on each current package.

  1. Dispense

    On clean, dry skin, dispense one pump from each chamber into your palm.

  2. Mix

    Mix the two formulas together before applying.

  3. Apply

    Apply evenly to the face and neck, morning and night. Allow 1–2 minutes to absorb before layering other products.

Inside the formulation.

The formulation includes ascorbic acid (vitamin C), d-tocopheryl acetate (vitamin E), sodium nitrite and hydroxyethylcellulose. Review the full formula and the current product label.

Complete ingredient list

Water , Ascorbic Acid (Vitamin C), Polysorbate 20 , Hydroxyethylcellulose , Sodium Nitrite , d-Tocopheryl Acetate (Vitamin E), Dehydroacetic Acid , Benzyl Alcohol , Sodium Sulfite , Sodium Metabisulfite , Tetrasodium EDTA .

Read the product label

View the original label images in the gallery above. Your current package is the reference for the formula and directions.

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Watch. Learn. Make it a routine.

Original N1O1 videos for this product. Choose a video to hear the explanation and see the routine.

Dr. Nathan Bryan - Nitric Oxide Activating Serum How To

Watch the original N1O1 demonstration.

Susan - N1o1 Nitric Oxide Activating Serum How to Use

Watch the original N1O1 demonstration.

Developed by the scientist behind N1O1.

Dr. Nathan S. Bryan’s work focuses on nitric oxide biochemistry. Explore the formulation, follow the directions and keep learning about the science behind your routine.

Hear from N1O1
Dr. Nathan S. Bryan, the scientist behind N1O1

Go deeper into the science.

Research references linked by N1O1 for this product. Individual studies have their own designs and populations; they are provided for further reading.

What customers report.

4.94 / 5.0 from 31 published customer reviews.

Questions before you start?

The essentials for Age-Defiance Activating Serum.

Why does the serum have two chambers?

The chambers keep the two formulas separate until application. Dispense one pump from each and mix in your palm.

When should I apply it?

N1O1 recommends morning and night on clean, dry skin. Allow 1–2 minutes for absorption before applying additional products.

How long does a bottle last?

The original instructions describe approximately 70 applications per bottle. Actual use depends on how much you dispense.

What if I notice redness or discomfort?

N1O1 describes a temporary pink flush after application. Stop use if you experience irritation or persistent discomfort and ask a healthcare professional for advice.

How do subscriptions work?

Choose Subscribe in the buy box to see the current price and delivery schedule. The selling plan and selected quantity are included when you add the product to your cart. Review the subscription terms at checkout.

What about shipping and returns?

Free shipping applies to orders over $75 in the continental US. Read the current policy for eligibility, timing and return instructions.

Read shipping & return terms

1 bottle$119.98

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