Mix at application
The two-chamber design keeps the formulas separate until you dispense them.






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The N1O1 dual-chamber serum keeps the two formulas separate until use. Mix them in your palm, then apply to the face and neck.
1 fl oz / 30 mL · dual-chamber bottle
The N1O1 dual-chamber serum keeps the two formulas separate until use. Mix them in your palm, then apply to the face and neck.
The two-chamber design keeps the formulas separate until you dispense them.
The original product page highlights vitamin C, vitamin E and sodium nitrite.
Allow 1–2 minutes for absorption before applying other products.
Build your routine, step by step. Follow the directions on each current package.
On clean, dry skin, dispense one pump from each chamber into your palm.
Mix the two formulas together before applying.
Apply evenly to the face and neck, morning and night. Allow 1–2 minutes to absorb before layering other products.
The formulation includes ascorbic acid (vitamin C), d-tocopheryl acetate (vitamin E), sodium nitrite and hydroxyethylcellulose. Review the full formula and the current product label.
Water , Ascorbic Acid (Vitamin C), Polysorbate 20 , Hydroxyethylcellulose , Sodium Nitrite , d-Tocopheryl Acetate (Vitamin E), Dehydroacetic Acid , Benzyl Alcohol , Sodium Sulfite , Sodium Metabisulfite , Tetrasodium EDTA .
View the original label images in the gallery above. Your current package is the reference for the formula and directions.
Back to the product galleryOriginal N1o1 videos for this product. Choose a video to hear the explanation and see the routine.
Watch the original N1o1 demonstration.
Watch the original N1o1 demonstration.
Dr. Nathan S. Bryan’s work focuses on nitric oxide biochemistry. Explore the formulation, follow the directions and keep learning about the science behind your routine.
Hear from N1o1
Research references linked by N1o1 for this product. Individual studies have their own designs and populations; they are provided for further reading.
4.94 / 5.0 from 31 published customer reviews.
The essentials for Age-Defiance Activating Serum.
The chambers keep the two formulas separate until application. Dispense one pump from each and mix in your palm.
N1O1 recommends morning and night on clean, dry skin. Allow 1–2 minutes for absorption before applying additional products.
The original instructions describe approximately 70 applications per bottle. Actual use depends on how much you dispense.
N1O1 describes a temporary pink flush after application. Stop use if you experience irritation or persistent discomfort and ask a healthcare professional for advice.
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Read shipping & return terms1 bottle$119.98
Sales, bundle pricing and new product drops, a few times a month.